C4ob02071e 357..360 ++
نویسندگان
چکیده
Stereoselectively labelled isotopomers of NAD(P)H are highly relevant for mechanistic studies of enzymes which utilize them as redox equivalents. Whereas several methods are firmly established for their generation in high diastereomeric purity by enzymatic methods, alternative methods have so far not been investigated. The article presents the stereoselective deuteration of NAD at the 4-position (90% de) of the pyridinium-ring by means of an artificial metalloenzyme. The artificial metalloenzyme consists of a biotinylated iridium cofactor embedded in streptavidin isoforms and the resulting constructs have been previously shown to be compatible with natural enzymes. Alternative methods for stereoselective NAD (P) reduction are expected to be of high interest for the mechanistic study of enzymes that accept NAD(P)H mimics and for the synthesis of structurally related fine chemicals.
منابع مشابه
The Inheritance of Resistance to the Danysz Bacillus in the Rat.
INTRODUC~ON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 337 Materialsandmethods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 Percentage mortalities due to varying numbers of bacteria.. . . . . . . . . . . . . . . . . . . . . . . . ....
متن کاملDouble Bruhat Cells and Total Positivity
0. Introduction 336 1. Main results 337 1.1. Semisimple groups 337 1.2. Factorization problem 338 1.3. Total positivity 339 1.4. Generalized minors 339 1.5. The twist maps 340 1.6. Formulas for factorization parameters 341 1.7. Total positivity criteria 343 1.8. Fundamental determinantal identities 343 2. Preliminaries 344 2.1. Involutions 344 2.2. Commutation relations 345 2.3. Generalized det...
متن کاملSyringocystadenoma papilliferum without an antecedent naevus sebaceous.
meningioma. Surg Neurol 1986; 25: 357 ^ 360. 4. Kendall B, Moseley I. Therapeutic embolization of the external carotid arterial tree. J Neurol Neurosurg Psychiatry 1977; 40: 937 ^ 950. 5. Chan RC, Thompson GB. Ischemic necrosis of the scalp after preoperative embolization of meningeal tumors. Neurosurgery 1984; 15: 76 ^ 81. 6. Dawber RPR, Ebling FJG, Wojnarowska FT. Disorders of hair. In: Champ...
متن کاملCare in use of phenazine methosulfate in the laboratory.
3. Soldin, S. J., and Hill, ,J. R., A rapid micromethod for measuring theophylline in serum by reversed-phase high-performance liquid chromatography. Clin. Biochem. 10, 74-77(1977). 4. Nelson, J. W., Cordry, A. L., Aron, C. C., and Baitell, R. A., Simplified micro-scale procedure for preparing samples for theophylline determination by liquid chromatography. Clin. Chem. 23, 124-126 (1977). 5. Pe...
متن کامل